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The coding sequence was replaced with a floxed neomycin resistance cassette and a modified coding sequence in which a G to T point mutation results in the amino acid substitution of aspartic acid for glutamic acid at position 160 (E160D). Cre-mediated recombination removed the selection cassette. The E160D mutation abolishes >90% of the 5' exonuclease (EXO) and gap-dependent endonuclease (GEN) activities but retains the flap-specific endonuclease (FEN) protein activity, representing mutations identified in human cancers. Western blot analysis revealed that protein expression is unaffected in MEF lysates from heterozygous and homozygous mutant mice. (J:231218)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
