Tg(Opalin-icre/ERT2)#Rjfl

The mouse was generated via bacterial artificial chromosome transgenesis using the Opalin genomic bacterial artificial chromosome RP24-510M10. Insertion of the iCreERT2-polyA-frt-Kanamycin-frt cassette was achieved by homologous recombination (with no loss of the genomic bacterial artificial chromosome) in BAC (RPCI24-510M10) immediately after the Opalin initiation codon ATG of the open reading frame of the Opalin sequence. The frt-kanamycin-frt was removed using flippase recombinase. Four founders were generated, three of which expressed the transgene. The one with highest expression was chosen for further experimentation. Line numbers were not indicated. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:229987)