Prm2^em1Hsc

CRISPR/Cas9-mediated genome editing generated a 97bp deletion in exon 1 leading to a frameshift that results in nonsense transcripts with a premature stop codon after amino acid 4. Western blot analysis of basic nuclear proteins isolated from epididymal sperm confirmed the absence of detectable mature protein in homozygous mutant males. (J:243626)