Ptchd1^{tm1a(KOMP)IcsOrl}

The modified vector was inserted at position 155587519 of Chromosome X upstream of exon 2. (Build GRCm38). The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, an SV40 polyA sequence, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 155587393. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:204739)