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RMCE on KH2 cells inserted into the 3'UTR a RNU6 promoter driving expression of a paired guide RNAs targeting murine Apc 12 bp immediately upstream of the NGG protoscpacer-adjacent motif (PAM) sequence followed by a tetracycline response element promoter driving inducible expression of a GFP report gene, IRES sequence, human codon optimized CRISPR-associated endonuclease nickase variant cas9D10A (Cas9n; derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) and polyadenylation sequence. (J:222595)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
