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The 166 kb C57BL/6J mouse bacterial artificial chromosome (BAC) from the RPCI23 library containing the entire Pax8 locus (and other genes) was modified by targeting a yellow fluorescent protein (YFP) coding sequence into the ATG start site of Pax8. This inserted a YFP fusion protein coding sequence, SV40 polyA signal, and a FRT-flanked Kanamycin resistance gene cassette. No other genes on the BAC were modified or deleted. Flp-mediated recombination removed the Kanamycin cassette. The BAC clone backbone vector (pBACe3.6), which contains the chloramphenicol resistance (CmR) gene, was not removed prior to microinjection and thus is retained in the transgenic animals. (J:264384)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
