Tg(Npy5r-itTA,Npy1r-Venus)25Rsp

In a single mouse bacterial artificial chromosome (BAC) bearing the neuropeptide Y receptor Y5 (Npy5r) and the neuropeptide Y receptor Y1 (Npy1r) genes in their native head-to-head orientation, separated by ~25 kb containing their 5' regulatory sequences, the Npy5r and Npy1r protein coding exons were replaced, respectively, with the coding sequences for "improved" tetracycline transactivator (itTA) protein and for the Venus enhanced yellow fluorescent protein followed by a polyadenylation signal and a frt site-flanked neomycin resistance cassette or a single frt site, respectively. Five transgenic mouse lines, designated 4, 25, 27, 35, and 39, were generated. Line 25, estimated by Southern blot analysis to carry six copies of the transgene, exhibited the greatest expression from both promoters. Venus fluorescence under the Y1R promoter is intense and widely present throughout the brain, essentially recapitulating published Npy1r expression, but with some discrepancies in the cerebral cortex and hippocampus. Immunohistochemical visualization of itTA-induced Cre recombinase expression from Tg(tetO-cre)LC1Bjd in double-transgenic mice demonstrates a Cre expression pattern similar to that reported for Npy5r that is repressible by doxycycline. (J:125957)