Exons 2 and 3 that encode for the GTPCH active site were flanked by loxP sites and an FRT flanked neo cassette was inserted in intron 2. Recombinase mediated recombination removed the neo cassette and exons 2 and 3. (J:219813)
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Exons 2 and 3 that encode for the GTPCH active site were flanked by loxP sites and an FRT flanked neo cassette was inserted in intron 2. Recombinase mediated recombination removed the neo cassette and exons 2 and 3. (J:219813)