A loxP site was inserted 600 bp upstream of exon 2 and an FRT flanked neo cassette and loxP site were inserted in intron 2 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exon 2. (J:218253)
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A loxP site was inserted 600 bp upstream of exon 2 and an FRT flanked neo cassette and loxP site were inserted in intron 2 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exon 2. (J:218253)