An FRT-flanked neomycin resistant cassette with a 5' loxP site was inserted upstream of exon 5. An additional loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the selection cassette and left exon 5 floxed. (J:225216)
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An FRT-flanked neomycin resistant cassette with a 5' loxP site was inserted upstream of exon 5. An additional loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the selection cassette and left exon 5 floxed. (J:225216)