Tg(hs1538-cre/ERT2,-GFP)1608Jlr

This transgene contains the human enhancer element hs1538, which is flanked on human Chr 14 by the MAP3K12 binding inhibitory protein 1 (MBIP) and surfactant associated 3 (SFTA3) genes, followed by a minimal Hsp68 promoter, the cre/ERT2 fusion gene, an internal ribosomal entry site (IRES), and a green fluorescent protein reporter gene. The fusion gene encodes Cre recombinase joined to a modified ligand-binding domain of the human estrogen receptor that preferentially binds tamoxifen or 4-hydroxytamoxifen, but not endogenous 17beta-estradiol; upon binding to tamoxifen, the cre/ERT2 protein is translocated to the nucleus. (J:94077)