A loxP site was inserted upstream of exon 5. An FRT-flanked selection cassette with a 3' loxP site were inserted downstream of exon 6. Flp-mediated recombination removed the selection cassette and left exons 5 and 6 floxed. (J:225221)
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A loxP site was inserted upstream of exon 5. An FRT-flanked selection cassette with a 3' loxP site were inserted downstream of exon 6. Flp-mediated recombination removed the selection cassette and left exons 5 and 6 floxed. (J:225221)