A loxP site was inserted upstream of exon 1. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 8. Cre-mediated recombination removed exons 1 through 8 and the selection cassette. (J:223048)
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A loxP site was inserted upstream of exon 1. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 8. Cre-mediated recombination removed exons 1 through 8 and the selection cassette. (J:223048)