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The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, an FRT-flanked tdTomato sequence (tdTomato is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), three copies of green fluorescent protein, and a Neo-polyA cassette. The three GFP sequences include emerald, TagGFP (Aequorea macrodactyla GFP), and hrGFP (Humanized Renilla reniformis GFP) variants. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:235123)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
