A loxP site was inserted 5' to exon 3 and a loxP site and an frt-flanked neomycin selection cassette was inserted 3' to exon 3. The neomcyin selection cassette was removed by Flp-mediated recombination. (J:222375)
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A loxP site was inserted 5' to exon 3 and a loxP site and an frt-flanked neomycin selection cassette was inserted 3' to exon 3. The neomcyin selection cassette was removed by Flp-mediated recombination. (J:222375)