Ret^tm1.2Ddg

Exons 14 and 15 and an FRT-flanked neo cassette are flanked by loxP sites. A missense mutation in exon 15 changes valine 805 to alanine. This mutation is silent but sensitizes the protein to chemical inhibition of its kinase activity. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 14 and 15 floxed. (J:126484)