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This transgene contains from 5' to 3', the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a loxP-flanked STOP cassette (beta-galactosidase/neomycin resistance cassette [beta-geo] with three copies of the SV40 polyA termination signal) and the 1.8 kbp human myelocytomatosis oncogene cDNA sequence (MYC; including exons 2 and 3 and polyA site). The 10.8 kbp transgene was linearized and transfected into 129S/SvEv-derived embryonic stem (ES) cells. An ES cell clone with a single-copy transgene integration was selected for injection into blastocysts. Constitutive beta-galactosidase (lacZ) expression is directed to multiple tissue types. Removal of the STOP cassette by Cre-mediated recombination results in removal of lacZ and expression of MYC. (J:114516)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
