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EN
A targeting vector was designed to insert a loxP site upstream of exon 1, cDNA encoding the sequence of exon 3 fused in frame to the endogenous exon 2, followed by an SV40 polyadenylation sequence bound to an FRT-flanked neomycin resistance (neo) cassette and a second loxP site. Duplicate exons 1-2 were inserted downstream of the second loxP site and upstream of the endogenous exon 3. The second exon 1 contains a mutation in amino acid 95 which alters the nucleotide sequence from CCG to CAC, resulting in an amino acid change from Proline to Histidine. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:221404)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
