A loxP site followed by Frt-flanked neo cassette were inserted upstream of exon 3 and another loxP site in intron 7. Flp-mediated recombination removed neo cassette, leaving exons 3 through 7 floxed. (J:223453)
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A loxP site followed by Frt-flanked neo cassette were inserted upstream of exon 3 and another loxP site in intron 7. Flp-mediated recombination removed neo cassette, leaving exons 3 through 7 floxed. (J:223453)