A loxP site was inserted upstream of exon 7 and a Frt-flanked neo cassette with 3' loxP sequence were inserted downstream of exon 7. Flp mediated recombination removed the neo cassette leaving loxP-flanked exon 7. (J:223478)
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A loxP site was inserted upstream of exon 7 and a Frt-flanked neo cassette with 3' loxP sequence were inserted downstream of exon 7. Flp mediated recombination removed the neo cassette leaving loxP-flanked exon 7. (J:223478)