A loxP site was inserted upstream of exon 4 and a FRT-flanked neomycin cassette with a 3' loxP site were inserted downstream of exon 5. Flp-mediated recombination removed the neo cassette leaving exons 4 and 5 floxed. (J:225609)
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A loxP site was inserted upstream of exon 4 and a FRT-flanked neomycin cassette with a 3' loxP site were inserted downstream of exon 5. Flp-mediated recombination removed the neo cassette leaving exons 4 and 5 floxed. (J:225609)