A loxP site was inserted in intron 6 and an FRT and loxP flanked pgk-neo cassette was inserted in intron 7 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 7 floxed. (J:217087)
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A loxP site was inserted in intron 6 and an FRT and loxP flanked pgk-neo cassette was inserted in intron 7 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 7 floxed. (J:217087)