A loxP site was inserted upstream of exon 1 and a pgk-neo cassette and loxP site were inserted downstream of exon 1 via homologous recombination. Cre mediated recombination removed exon 1 and the neo cassette. (J:174192)
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A loxP site was inserted upstream of exon 1 and a pgk-neo cassette and loxP site were inserted downstream of exon 1 via homologous recombination. Cre mediated recombination removed exon 1 and the neo cassette. (J:174192)