A loxP site and FRT flanked Ncol-pgk-neo cassette were inserted upstream of exon 3 and a loxP site was inserted at the end of exon 3 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:216751)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count