A loxP site and FRT flanked Ncol-pgk-neo cassette were inserted upstream of exon 3 and a loxP site was inserted at the end of exon 3 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:216751)
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A loxP site and FRT flanked Ncol-pgk-neo cassette were inserted upstream of exon 3 and a loxP site was inserted at the end of exon 3 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:216751)