Malt1^tm1.1Math

A TGTCGG to GCCAGA mutation at nucleotides 168-173 of exon 11 encoding a C472A substitution was introduced and an FRT flanked neo cassette was inserted in intron 10 via homologous recombination. Flp mediated recombination removed the neo cassette. Immunoblot analysis indicates expression of the mutant protein at levels similar to wild-type protein. The C472A mutation produces a catalytically inactive protein that fails to cleave multiple substrates (Bcl-10, Regnase-1, RelB, and CYLD). (J:216675)