The human NGF gene is targeted to thecal-interstitial cells by the mouse 17alpha-hydroxylase promoter. RNase protection assays confirmed ovarian mRNA expression and to a much lesser extent, expression in the adrenal gland, in the kidney and the liver.Two lines, 2380 and 2456, were generated and exhibited similar phenotypes. Line 2456 was studied further. (J:158186)