A loxP site was inserted upstream of the transcription start site and a loxP-FRT-neo-FRT cassette was inserted in intron 1 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:216471)
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A loxP site was inserted upstream of the transcription start site and a loxP-FRT-neo-FRT cassette was inserted in intron 1 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:216471)