Nlgn3^tm4.1Sud

The following elements were inserted into intron 1: an FRT site, a duplicate of the 5' end of the ATG translation start site-containing exon 2, a full-length Nlgn3 cDNA (incorporating mutations corresponding to Nlgn1 "5/32" (L399A, N400A, D402N, E297A, and K306A) that abolish neurexin binding) fused to the cytoplasmic monomeric green fluorescent protein mVenus gene (lacking splice site A), an F3 FRT site, a neomycin resistance gene cassette, a tandem FRT/F3 FRT site, and a loxP511 site. A second loxP511 site was inserted into intron 3 to allow for conditional cre-mediated deletion of exons 2 and 3. This conditional knockout allele, expressing the wild-type peptide, was created through subsequent flp-mediated recombination via the FRT sites (which removes the the neo cassette and knockin transcript and leaves exons 2-3 floxed). (J:214636)