A loxP site was inserted upstream of exon 2 and a loxP and FRT flanked neo cassette was inserted downstream of exon 3 via homologous recombination. Flp-meditated recombination removed the neo cassette leaving exons 3 and 4 floxed. (J:214740)
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A loxP site was inserted upstream of exon 2 and a loxP and FRT flanked neo cassette was inserted downstream of exon 3 via homologous recombination. Flp-meditated recombination removed the neo cassette leaving exons 3 and 4 floxed. (J:214740)