Tg(NES/TK-PDGFB,-lacZ)312Kfn

This transgene consists of the human nestin second intron enhancer joined to a basic thymidine kinase promoter and the human PDGFB cDNA (without the 5' and 3' untranslated regions) followed by an internal ribosomal entry site (IRES) and a lacZ reporter gene. The minimal nestin enhancer element is specific for embryonic expression but will not drive adult expression of PDGF-B. Three lines were made: 310, 312, and 319. Beta-galactosidase assays show that the transgene is expressed in the developing CNS, with high expression at E10.5, limited expression at E17.5 and no expression at P0. In the retina, transgene expression commences on E17.5 and attains maximal level between P1 and P15, before declining on P20. (J:165035)