A loxP site was inserted upstream of exon 2 and an FRT flanked pgk-gb2-neo cassette and loxP site were inserted downstream of exon 4 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 2-4. (J:214321)
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A loxP site was inserted upstream of exon 2 and an FRT flanked pgk-gb2-neo cassette and loxP site were inserted downstream of exon 4 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 2-4. (J:214321)