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The transgenic construct comprised the 130-kb RPCI PAC clone 204D22, containing the human adenylate cyclase activating polypeptide 1 receptor 1 (ADCYAP1R1; also called PAC1) gene, modified by insertion of an internal ribosomal entry site (IRES) followed by the lacZ coding sequence (IRES-lacZ-p[A] cassette) into the 3' untranslated region (3' UTR) downstream of the human gene's polyadenylation signal. Semi-quantitative PCR of genomic DNA from heterozygous mice determined that transgenic line 1 (also called A149) carries six copies of the transgene. RT-PCR of RNA from embryonic day 10.5 (E10.5) embryos revealed a high level of transgene expression. (J:111714)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
