Exons 3-5 were floxed by inserting a floxed neo cassette and an FRT flanked neo cassette with a single loxP site via BAC recombineering. Both selection cassettes were removed by recombinase mediated recombination. (J:213786)
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Exons 3-5 were floxed by inserting a floxed neo cassette and an FRT flanked neo cassette with a single loxP site via BAC recombineering. Both selection cassettes were removed by recombinase mediated recombination. (J:213786)