A loxP site was inserted in intron 1 and an FRT and loxP flanked neo cassette was inserted in intron 5 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 2-5. (J:213786)
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A loxP site was inserted in intron 1 and an FRT and loxP flanked neo cassette was inserted in intron 5 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 2-5. (J:213786)