An FRT-flanked neo cassette followed by a loxP site was inserted intron 5. A second loxP site was inserted in intron 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 3 through 5 floxed. (J:179270)
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An FRT-flanked neo cassette followed by a loxP site was inserted intron 5. A second loxP site was inserted in intron 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 3 through 5 floxed. (J:179270)