Exons 5 and 6 were replaced with a fused exon 5-6 lacking the HD1 domain and an FRT and loxP flanked neo cassette was inserted intron 4 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:213694)
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Exons 5 and 6 were replaced with a fused exon 5-6 lacking the HD1 domain and an FRT and loxP flanked neo cassette was inserted intron 4 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:213694)