A loxP site was inserted in intron 3 and an FRT flanked pgk-neo cassette and loxP site were inserted in intron 5 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exons 4 and 5 floxed. (J:221221)
查看原文 参与反馈
A loxP site was inserted in intron 3 and an FRT flanked pgk-neo cassette and loxP site were inserted in intron 5 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exons 4 and 5 floxed. (J:221221)