A loxP site was inserted upstream of exon 3. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the selection cassette and left exons 3 through 5 floxed. (J:215353)
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A loxP site was inserted upstream of exon 3. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the selection cassette and left exons 3 through 5 floxed. (J:215353)