Hipk4^{tm1b(KOMP)Mbp}

Cre-mediated excision of the parental Hipk4tm1a(KOMP)Mbp allele resulted in the removal of the promoter-driven neomycin selection cassette and critical exons (exons 2 and 3) leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. PCR of tail-derived genomic DNA and Western blot analyses of whole testis lysates confirmed the absence of functional gene products in homozygous male mice. (J:204739, J:290279)