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The mouse bacterial artificial chromosome (BAC) RP24-306A6 containing the entire Pvalb gene, was modified first by a BAC trimming method to remove the Ift27 gene coding region. The second BAC recombineering round inserted the tdTomato-WPRE-BGHpA (bovine growth hormone polyadenylation signal)-FRT-NEO-FRT vector directly downstream of the Pvalb initiation start codon. The FRT site flanked NEO was then removed by transient FLP recombinase expression. Correctly targeted BAC DNA was used as the source for the transgene, which was linearized to remove it from the vector. (J:101977, J:232250)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
