An FRT flanked neo cassette was inserted in intron 2 followed by a loxP site. A second loxP site was inserted upstream of exon 2. The neo cassette was subsequently excised by frt expression leaving exon 2 flanked by loxP sites. (J:212351)
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An FRT flanked neo cassette was inserted in intron 2 followed by a loxP site. A second loxP site was inserted upstream of exon 2. The neo cassette was subsequently excised by frt expression leaving exon 2 flanked by loxP sites. (J:212351)