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The targeting vector consisting of a loxP site, exon 2, a frt-flanked neomycin cassette, and a loxP site was inserted into the locus. Cre-mediated recombination deleted exon 2 and the neomycin cassette. DNA blot analysis confirmed the presence of the disrupted allele and RT-PCR confirmed ablation of the full-length mRNA expression. Western blot confirmed a complete absence of protein, indicating this is a functionally null allele. (J:218010)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
