An FRT flanked pgk-neo cassette and loxP site were inserted upstream of exon 4 and a loxP site was inserted downstream of exon 12 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:208414)
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An FRT flanked pgk-neo cassette and loxP site were inserted upstream of exon 4 and a loxP site was inserted downstream of exon 12 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:208414)