Exon 3 was flanked with loxP sites and FRT (neo) and F3 (puro) flanked selection cassettes were inserted into intons 2 and 3, respectively. Flp mediated recombination removed the selection cassettes. (J:207485)
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Exon 3 was flanked with loxP sites and FRT (neo) and F3 (puro) flanked selection cassettes were inserted into intons 2 and 3, respectively. Flp mediated recombination removed the selection cassettes. (J:207485)