Two transgenic constructs were coinjected. The first construct has the CAG promoter fused to a mitochondrial import sequence (Atp5g1) driving expression of RFP (DsRed2). The second construct has the acrosin promoter with a proacrosin signal sequence driving expression of GFP. The sperm head is green prior to the acrosome reaction, after acrosome reaction GFP expression is lost. RFP expression is seen in the mitochondria in the midpiece of the sperm. (J:216056)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6 x DBA/2
--
Insertion
--
--
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34

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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