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An IRES-GFP-FRT-PGK-neo-FRT-loxP cassette was inserted immediately downstream of the stop codon in exon 12, along with a loxP site in intron 10. The PGK-neo cassette was removed via Flp-mediated recombination, leaving exons 11-12 and the IRES-GFP floxed. Expression of GFP was confirmed by flow cytometry and live cell imaging in megakaryocyte progenitor cells, megakaryoctyes, and LSK cells. (J:208120)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
