A targeting vector was designed to insert a loxP site upstream of exon 2 followed by a second loxP site followed by a frt-flanked neomycin resistance (neo) cassette downstream of exon 2 of the gene. Flp-mediated recombination removed the neo cassette leaving exon 2 floxed. (J:196291)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count