Jagn1^{tm1c(EUCOMM)Hmgu}

The L1L2_Bact_P cassette was inserted at position 113423780 of Chromosome 6 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neo cassette and left exon 2 floxed. (J:214828)