Tg(MMTV-Akt1)1173Stma

This transgene expresses a constitutively active mutant of Akt in the mammary glands of mice under the control of the MMTV promoter, which allows expression of the transgene in the epithelial cells of the mammary gland. The active mutant of Akt (myr-Akt) used in these studies contains an amino-terminal myristoylation sequence from c-src fused to the coding sequence of Akt1. This directs the fusion protein to the plasma membrane where it can be constitutively activated after phosphorylation of Thr308 and Ser473. A hemagglutinin (HA) epitope tag at the carboxyl-terminal end of the protein was also included to allow for detection of expression by immunoblotting. In addition to line 1173, the lower expressing line 1176 was also created. (J:69612)