S1pr1^{tm3.1(tTA,-Arrb2)Rlp}

A targeting vector was designed with two fusion proteins separated by an internal ribosome entry site (IRES). One fusion protein contains a tobacco etch virus (TEV) protease recognition sequence (tevs) fused to a tetracycline-regulated transactivator (tTA or "Tet-Off") sequence. The second contains a murine arrestin, beta 2 (Arrb2) gene fused to the TEV protease. This construct was inserted at the 3 end of exon 2 of the sphingosine-1-phosphate receptor 1 (S1pr1) gene and was followed by a polyadenylation sequence and a loxP-flanked neomycin (neo) resistance cassette. Cre-mediated recombination removed the selection cassette. (J:212772)